MEF2D Antibody | MyBioSource MBS7050075 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MEF2D Antibody

catalog :

MBS7050075

quantity :

0.05 mg

price :

160 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot. Positive WB detected in: Hela whole cell lysate,HepG2 whole cell lysate,A549 whole cell lysate,Mouse heart tissue. All lanes: MEF2D antibody at 2.7ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56,57,51,50 KDa. Observed band size: 56 KDa.

image 2 :

IHC image of MBS7050075 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

image 3 :

IHC image of MBS7050075 diluted at 1:400 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

product information

catalog number :

MBS7050075

products type :

Antibody

products full name :

MEF2D Antibody

products short name :

[MEF2D]

products name syn :

[Myocyte-specific enhancer factor 2D; MEF2D]

other names :

[myocyte-specific enhancer factor 2D isoform 2; Myocyte-specific enhancer factor 2D; myocyte-specific enhancer factor 2D; myocyte enhancer factor 2D]

products gene name :

[MEF2D]

other gene names :

[MEF2D; MEF2D]

uniprot entry name :

MEF2D_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

514

purity :

>95%, Protein G purified

form :

Liquid

storage stability :

Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1: 500-1: 5000. IHC: 1: 20-1: 200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Recombinant Human Myocyte-specific enhancer factor 2D protein (299-509AA). Species: Human. Research Area: Neuroscience

other info2 :

Storage Buffer: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. Conjugation: Non-conjugated. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-13921

products description :

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis (By similarity).

ncbi gi num :

410442515

ncbi acc num :

NP_001258558.1

ncbi gb acc num :

NM_001271629.1

uniprot acc num :

Q14814

ncbi mol weight :

49,856 Da

ncbi pathways :

Adipogenesis Pathway (198832); B Cell Receptor Signaling Pathway (198909); CDO In Myogenesis Pathway (1270336); Cell Differentiation - Index Pathway (1458245); Circadian Clock Pathway (1269871); Developmental Biology Pathway (1270302); EGF/EGFR Signaling Pathway (198782); Mitochondrial Biogenesis Pathway (1268839); Myogenesis Pathway (1270335); Organelle Biogenesis And Maintenance Pathway (1268838)

ncbi summary :

This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

uniprot summary :

MEF2D: transcription factor which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. May be involved in muscle-specific and/or growth factor-related transcription. Six splice-variant isoforms have been described. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 1q12-q23. Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus. Molecular Function: histone deacetylase binding; protein binding; transcription activator binding. Biological Process: adult heart development; muscle development; positive regulation of transcription from RNA polymerase II promoter

size1 :

0.05 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

235

more info or order :

MyBioSource product webpage