ELISA/assay

Human cholecystokinin octapeptide, CCK-8 ELISA Kit

MBS705001

96 Strip Wells

850 USD

ELISA Kit

Human cholecystokinin octapeptide, CCK-8 ELISA Kit

cholecystokinin octapeptide, CCK-8

Human cholecystokinin octapeptide; CCK-8 ELISA Kit; cholecystokinin octapeptide; CCK-8

CCK-8

Human

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma. Detection Range: 0.57 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.357 ng/ml

Sample Volume: 50-100ul

Introduction: Cholecystokinin(CCK) plays a key role in facilitating digestion within the small intestine. It is secreted from mucosal epithelial cells in the first segment of the small intestine (duodenum), and stimulates delivery into the small intestine of digestive enzymes from the pancreas and bile from the gallbladder. Cholecystokinin is also produced by neurons in the enteric nervous system, and is widely and abundantly distributed in the brain. Cholecystokinin and gastrin are highly similar peptides. Like gastrin, cholecystokinin is a linear peptide that is synthesized as a preprohormone, then proteolytically cleaved to generate a family of peptides having the same carboxy ends. Full biologic activity is retained in CCK-8 (8 amino acids), but peptides of 33, 38 and 59 amino acids are also produced. In all of these CCK peptides, the tyrosine seven residues from the end is sulfated, which is necessary for activity. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCK-8. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated monoclonal antibody preparation specific for CCK-8. and incubated. Then substrate solution A and B are added to each well. Only those wells that contain CCK-8., HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CCK-8. in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

850 USD