ELISA/assay

Rat glucose transporter 4, GLUT4 ELISA Kit

MBS704978

96 Strip Wells

850 USD

ELISA Kit

Rat glucose transporter 4, GLUT4 ELISA Kit

solute carrier family 2 (facilitated glucose transporter) , member 4

Rat glucose transporter 4 (GLUT4) ELISA Kit; GLUT4; glucose transporter 4; insulin-responsive glucose transporter type 4; solute carrier family 2 (facilitated glucose transporter); member 4

solute carrier family 2, facilitated glucose transporter member 4; Solute carrier family 2, facilitated glucose transporter member 4; solute carrier family 2, facilitated glucose transporter member 4; GLUT-4; solute carrier family 2 member 4; solute carrier family 2 , member 4; insulin responsive glucose transporter; Glucose transporter 4 insuline-responsive; Glucose transporter 4, insuline-responsive; glucose transporter type 4, insulin-responsive; solute carrier family 2 (facilitated glucose transporter), member 4; Glucose transporter type 4, insulin-responsive; GLUT-4

SLC2A4

Slc2a4; Slc2a4; Glut4; Glut-4; Glut4; GLUT-4

GTR4_RAT

Rat

509

This assay has high sensitivity and excellent specificity for detection of rat GLUT4. No significant cross-reactivity or interference between rat GLUT4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of rat glucose transporter 4 (GLUT4) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 0.16 ng/ml-10 ng/ml.

Sensitivity: The minimum detectable dose of rat GLUT4 is typically less than 0.04 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GLUT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLUT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLUT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLUT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6980958

NP_036883.1

NM_012751.1

P19357

54,896 Da

AMPK Signaling Pathway 989832!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83485!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198479!!Developmental Biology Pathway 1009870!!Disease Pathway 1010484!!Facilitative Na+-independent Glucose Transporters Pathway 1009984!!FoxO Signaling Pathway 921494!!Glucose Transport Pathway 1009981

transports glucose; may play a role in insulin response [RGD, Feb 2006]

Function: Insulin-regulated facilitative glucose transporter.

96 Strip Wells

850 USD