catalog number :
MBS704973
products type :
ELISA Kit
products full name :
Mouse High density lipoprotein cholesterol, HDL-C ELISA Kit
products short name :
High density lipoprotein cholesterol, HDL-C
products name syn :
Mouse High density lipoprotein cholesterol; HDL-C ELISA kit; High density lipoprotein cholesterol; HDL-C
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse HDL-C. No significant cross-reactivity or interference between mouse HDL-C and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 ng/ml-500 ng/ml. Sensitivity: 1.95 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HDL-C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HDL-C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HDL-C is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HDL-C bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells