catalog number :
MBS704965
products type :
ELISA Kit
products full name :
Pig Angiopoietin 1, ANG-1 ELISA Kit
products short name :
angiopoietin 1
products name syn :
Pig Angiopoietin 1; ANG-1 ELISA Kit; AGP1; AGPT; ANG1; ; angiopoietin 1
other names :
angiopoietin-1; Angiopoietin-1; angiopoietin-1; ANG-1
products gene name :
ANGPT1
other gene names :
ANGPT1; ANGPT1; AGPT; ANG-1
uniprot entry name :
ANGP1_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of Pig ANGPT1. No significant cross-reactivity or interference between Pig ANGPT1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Detection Range: 8 ng/ml-200 ng/ml. Sensitivity: 5 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with a goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated ANG-1 and antibody preparation specific for ANG-1 and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ANG-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_999124.1
ncbi gb acc num :
NM_213959.1
ncbi mol weight :
57,413 Da
ncbi pathways :
HIF-1 Signaling Pathway (695240); PI3K-Akt Signaling Pathway (692249); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (869519); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (869517); Rheumatoid Arthritis Pathway (200307); Rheumatoid Arthritis Pathway (200269)
uniprot summary :
Function: Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development
size4 :
10x96-Strip-Wells