ELISA/assay

Human gamma glutamyl transpeptidase, GGT ELISA Kit

MBS704964

48-Strip-Wells

565 USD

ELISA Kit

Human gamma glutamyl transpeptidase, GGT ELISA Kit

gamma-glutamyltransferase 1

Human gamma glutamyl transpeptidase; GGT ELISA Kit; CD224; D22S672; D22S732; GGT; GTG; MGC96892; MGC96904; MGC96963; OTTHUMP00000028921; OTTHUMP00000159078; gamma-glutamyl transpeptidase; glutamyl transpeptidase; gamma-glutamyltransferase 1

gamma-glutamyltranspeptidase 1; Gamma-glutamyltranspeptidase 1; gamma-glutamyltranspeptidase 1; glutathione hydrolase 1; leukotriene-C4 hydrolase; gamma-glutamyltransferase 1; Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain

GGT1

GGT1; GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732; GGT; GGT 1

GGT1_HUMAN

Human

569

This assay has high sensitivity and excellent specificity for detection of Human GGT1. No significant cross-reactivity or interference between Human GGT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Amino-acid biosynthesis and metabolism. Protein Biological Process 3: Glutathione biosynthesis

Introduction: Gamma-glutamyl transferase (GGT or GGTP, or Gamma-GT) is involved in the transfer of amino acids across the cellular membrane. It is also involved in glutathione metabolism by transferring the glutamyl moiety to a variety of acceptor molecules including water, certain L-amino acids and peptides. Leaving the cysteine product to preserve intracellular homeostasis of oxidative stress. GGT is found on the cell surface on all cells, with particularly high concentrations in the liver, bile ducts, and kidney. The enzyme is also present in other tissues, such as the epididymis. Elevated GGT is a marker of recent alcohol misuse. It may indicate excess alcohol consumption up to 3 or 4 weeks prior to the test. It is also used as a diagnostic marker for other types of liver damage or conditions attributable to other causes unrelated to alcohol abuse. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GGT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GGT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GGT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GGT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

572152963

NP_001275762.1

NM_001288833.1

P19440

61,410 Da

Aflatoxin Activation And Detoxification Pathway (1016173); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Biological Oxidations Pathway (105698); Cyanoamino Acid Metabolism Pathway (82970); Cyanoamino Acid Metabolism Pathway (339); Eicosanoid Synthesis Pathway (198888); Glutathione Conjugation Pathway (105727); Glutathione Metabolism Pathway (82973)

The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]

Function: Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide, cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Isoform 3 seems to be inactive.7 PublicationsManual assertion based on experiment in:Ref.7. Catalytic activity: A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.8 PublicationsManual assertion based on experiment in:Ref.15. Enzyme regulation: Activated by autocatalytic cleavage.1 PublicationManual assertion based on experiment in:Ref.24

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150