catalog number :
MBS704958
products type :
ELISA Kit
products full name :
Rat Neuroglobin, NGB ELISA Kit
products short name :
[neuroglobin]
products name syn :
[Rat Neuroglobin; NGB ELISA Kit; neuroglobin]
other names :
[neuroglobin; Neuroglobin; neuroglobin]
products gene name :
[NGB]
other gene names :
[NGB; NGB]
uniprot entry name :
NGB_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat NGB. No significant cross-reactivity or interference between rat NGB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 ng/ml -1000 ng/ml. Sensitivity: The minimum detectable dose of rat NGB is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGB bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075602.1
ncbi gb acc num :
NM_001082133.2
ncbi mol weight :
17,040 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells