ELISA/assay

Rat Interleukin 3, IL-3 ELISA Kit

MBS704953

48-Strip-Wells

395 USD

ELISA Kit

Rat Interleukin 3, IL-3 ELISA Kit

interleukin 3 (colony-stimulating factor, multiple)

Rat Interleukin 3; IL-3 ELISA KIT; IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF; P-cell stimulating factor; hematopoietic growth factor; interleukin 3; mast-cell growth factor; multilineage-colony-stimulating factor; interleukin 3 (colony-stimulating factor; multiple)

Interleukin-3; Interleukin-3; interleukin-3; IL-3; MCGF; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor; interleukin 3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor

IL3

Il3; Il3; Il-3; IL-3; MCGF

IL3_RAT

Rat

166

This assay has high sensitivity and excellent specificity for detection of Rat IL3. No significant cross-reactivity or interference between Rat IL3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: 0.78 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

124334

P04823.1

P04823

18,631 Da

Apoptosis Pathway (83452); Apoptosis Pathway (470); Asthma Pathway (83508); Asthma Pathway (532); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198521); Fc Epsilon RI Signaling Pathway (83474); Fc Epsilon RI Signaling Pathway (493); Hematopoietic Cell Lineage Pathway (83470)

cytokine that functions as a multi-lineage haemopoietic growth regulator [RGD, Feb 2006]

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.

48-Strip-Wells

395 USD

96-Strip-Wells

550

5x96-Strip-Wells

2145

10x96-Strip-Wells

3995