ELISA/assay

Chicken Vascular Endothelial cell Growth Factor A, VEGF-A ELISA Kit

MBS704938

48-Strip-Wells

565 USD

ELISA Kit

Chicken Vascular Endothelial cell Growth Factor A, VEGF-A ELISA Kit

vascular endothelial growth factor A

Chicken Vascular Endothelial cell Growth Factor A (VEGF-A) ELISA KIT; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor; vascular endothelial growth factor A

vascular endothelial growth factor A isoform 2; Vascular endothelial growth factor A; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; vascular endothelial growth factor A; Vascular permeability factor; VPF

VEGFA

VEGFA; VEGFA; VEGF; VEGF; VEGF-A; VPF

VEGFA_CHICK

Chicken

192

This assay has high sensitivity and excellent specificity for detection of chicken VEGF-A. No significant cross-reactivity or interference between chicken VEGF-A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of chicken VEGF-A is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound in the initial step. The color development is stopped and the intensity of the color is measured.

160358853

NP_001103825.1

NM_001110355.1

P67964

25,203 Da

Cytokine-cytokine Receptor Interaction Pathway (119307); Cytokine-cytokine Receptor Interaction Pathway (460); Focal Adhesion Pathway (119312); Focal Adhesion Pathway (478); Id Signaling Pathway (199059); VEGF Signaling Pathway (83971); VEGF Signaling Pathway (477); MTOR Signaling Pathway (83965); MTOR Signaling Pathway (469)

Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150