ELISA/assay

Mouse transferrin receptor, TFR ELISA Kit

MBS704937

48-Strip-Wells

590 USD

ELISA Kit

Mouse transferrin receptor, TFR ELISA Kit

transferrin receptor (p90, CD71)

Mouse transferrin receptor; TFR ELISA Kit; CD71; TFR; TFR1; TRFR; transferrin receptor; transferrin receptor (p90; CD71)

transferrin receptor protein 1; Transferrin receptor protein 1; transferrin receptor protein 1; mammary tumor virus receptor 1; transferrin receptor; CD_antigen: CD71

TFRC

Tfrc; Tfrc; TR; TFR; p90; CD71; TFR1; Trfr; Mtvr1; Mtvr-1; AI195355; AI426448; AU015758; 2610028K12Rik; E430033M20Rik; Trfr; TR; TfR; TfR1; Trfr

TFR1_MOUSE

Mouse

763

This assay has high sensitivity and excellent specificity for detection of Mouse TFRC. No significant cross-reactivity or interference between Mouse TFRC and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Endocytosis

For Sample For the quantitative determination of mouse transferrin receptor (TFR) concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

11596855

NP_035768.1

NM_011638.4

Q62351

85,731 Da

Clathrin Derived Vesicle Budding Pathway (1000294); Endocytosis Pathway (102286); Endocytosis Pathway (102181); Golgi Associated Vesicle Biogenesis Pathway (1000295); HIF-1 Signaling Pathway (695223); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Iron Uptake And Transport Pathway (1001366); Membrane Trafficking Pathway (1000288); Phagosome Pathway (153914)

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis. [provided by RefSeq, Mar 2010]

Function: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system 1 PublicationManual assertion based on experiment in:Ref.5

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440