Chicken Ghrelin ELISA Kit | MyBioSource MBS704912 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Chicken Ghrelin ELISA Kit

catalog :

MBS704912

quantity :

48-Strip-Wells

price :

605 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704912

products type :

ELISA Kit

products full name :

Chicken Ghrelin ELISA Kit

products short name :

Ghrelin

products name syn :

Chicken Ghrelin ELISA Kit; Ghrelin

other names :

ghrelin; Ghrelin; Ghrelin

uniprot entry name :

E7CCH4_HUMAN

reactivity :

Chicken

sequence length :

117

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 25pg/ml-400pg/ml. Sensitivity: 12.5pg/ml

other info2 :

Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ghrelin. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated ghrelin. A competitive inhibition reaction is launched between ghrelin (Standards or samples) and Biotin-conjugated ghrelin with the pre-coated antibody specific for ghrelin. The more amount of ghrelin in samples, the less antibody bound by Biotin-conjugated ghrelin. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of ghrelin in the sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

304435649

ncbi acc num :

ADM33790.1

uniprot acc num :

E7CCH4

ncbi mol weight :

12,992 Da

ncbi pathways :

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

ncbi summary :

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

uniprot summary :

ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach. Protein type: Cell development/differentiation; Apoptosis; Secreted, signal peptide; Secreted; Cell cycle regulation. Chromosomal Location of Human Ortholog: 3p26-p25. Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region. Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity. Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; decidualization; positive regulation of multicellular organism growth; negative regulation of insulin secretion; positive regulation of adrenocorticotropic hormone secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; positive regulation of insulin secretion; glucose metabolic process; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; negative regulation of inflammatory response; cartilage development; actin polymerization and/or depolymerization; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis. Disease: Obesity

size1 :

48-Strip-Wells

price1 :

605 USD

size2 :

96-Strip-Wells

price2 :

870

size3 :

5x96-Strip-Wells

price3 :

2950

size4 :

10x96-Strip-Wells

price4 :

5535

more info or order :

MyBioSource product webpage