ELISA/assay

Human Creatine Kinase MB isoenzyme, CK-MB ELISA Kit

MBS704909

96 Strip Wells

850 USD

ELISA Kit

Human Creatine Kinase MB isoenzyme, CK-MB ELISA Kit

Creatine Kinase MB isoenzyme, CK-MB

Human Creatine Kinase MB isoenzyme; CK-MB ELISA Kit; Creatine Kinase MB isoenzyme; CK-MB

creatine kinase M-type; Creatine kinase M-type; creatine kinase M-type; creatine kinase M chain; creatine kinase, muscle; Creatine kinase M chain; M-CKCleaved into the following chain:Creatine kinase M-type, N-terminally processed

CK-MB

CKM; CKM; CKMM; M-CK; CKMM

KCRM_HUMAN

Human

381

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, urine, tissue homogenates. Detection Range: 0.9 ng/ml-60 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.22 ng/ml

Sample Volume: 50-100ul

For Sample: For the quantitative determination of human creatine kinase MB isoenzyme (CK-MB) concentrations in serum, plasma, urine. Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CK-MB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CK-MB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CK-MB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CK-MB bound in the initial step. The color development is stopped and the intensity of the color is measured.

21536288

NP_001815.2

NM_001824.4

P06732

43,101 Da

Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!Creatine Metabolism Pathway 106192!!Creatine Pathway 413359!!Creatine Pathway 468234!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Amino Acids And Derivatives Pathway 106169!!Regulation Of Retinoblastoma Protein Pathway 137916!!Urea Cycle And Metabolism Of Amino Groups Pathway 198758

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Catalytic activity: ATP + creatine = ADP + phosphocreatine.

96 Strip Wells

850 USD