ELISA/assay

Human receptor-binding cancer antigen expressed on SiSo cells, RCAS1 ELISA Kit

MBS704886

96 Strip Wells

850 USD

ELISA Kit

Human receptor-binding cancer antigen expressed on SiSo cells, RCAS1 ELISA Kit

estrogen receptor binding site associated, antigen, 9

Human receptor-binding cancer antigen expressed on SiSo cells; RCAS1 ELISA kit; EB9; PDAF; RCAS1; cancer associated surface antigen; estrogen receptor binding site associated antigen 9; estrogen receptor binding site associated; antigen; 9

receptor-binding cancer antigen expressed on SiSo cells; Receptor-binding cancer antigen expressed on SiSo cells; receptor-binding cancer antigen expressed on SiSo cells; cancer-associated surface antigen RCAS1; estrogen receptor-binding fragment-associated gene 9 protein; estrogen receptor binding site associated, antigen, 9; Cancer-associated surface antigen RCAS1; Estrogen receptor-binding fragment-associated gene 9 protein

EBAG9

EBAG9; EBAG9; EB9; PDAF; RCAS1

RCAS1_HUMAN

Human

213

This assay has high sensitivity and excellent specificity for detection of Human EBAG9. No significant cross-reactivity or interference between Human EBAG9 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum and tissue homogenates. Detection Range: 0.78 U/ml-50 U/ml. Detection Wavelength: 450 nm. Sensitivity: 0.195 U/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis

Introduction: Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a human tumor-associated antigen that induces cell cycle arrest and/or apoptosis in RCAS1 receptor-expressing immune cells. RCAS1 has been identified by means of a monoclonal antibody on the human uterine cervical adenocarcinoma cell line SiSo. RCAS1 is identical with the human estrogen-responsive gene product EBAG-9 (ER-binding fragment-associated antigen-9). RCAS1 is expressed at a high frequency in human uterine and ovarian cancer cells as well as in other mammalian cancer cells. High expression of RCAS1 has been shown to correlate significantly with tumour progression and predicted poor outcome in gallbladder cancer. RCAS1 is detected in nearly 100 % of gastric carcinomas and is strictly associated with tumor progression of gastric cancer. RCAS1 is highly expressed also in extramammary Paget's disease and in advanced stage squamous cell carcinoma of the skin. Ohshima et al have reported that RCAS1 is expressed in Reed-Sternberg cells in association with Epstein-Barr virus infection and that RCAS1 rather than FAS ligand allows these cells to evade host immune responses. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RCAS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for RCAS1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain RCAS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RCAS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

525459422

NP_001265867.1

NM_001278938.1

O00559

24,377 Da

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]

Function: May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.3 PublicationsManual assertion based on experiment in:Ref.8

96 Strip Wells

850 USD