catalog number :
MBS704873
products type :
ELISA Kit
products full name :
Mouse 5-Hydroxytryptamine, 5HT ELISA Kit
products short name :
5-Hydroxytryptamine, 5HT
products name syn :
Mouse 5-Hydroxytryptamine; 5HT ELISA Kit; 5-Hydroxytryptamine; 5HT
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse 5HT . No significant cross-reactivity or interference between mouse 5HT and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 250 pg /ml -4000 pg/ml. Sensitivity: The minimum detectable dose of mouse 5HT is typically less than 125 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse 5HT concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5HT. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated 5HT. The competitive inhibition reaction is launched between with HRP-conjugated 5HT and 5HT in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of 5HT in the sample. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells