catalog number :
MBS704829
products type :
ELISA Kit
products full name :
Human osteonectin, ON ELISA Kit
products short name :
sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2
products name syn :
Human osteonectin; ON ELISA Kit; FLJ97039; testican-2; ; sparc/osteonectin; cwcv and kazal-like domains proteoglycan (testican) 2
other names :
testican-2 isoform 1; Testican-2; testican-2; SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 2; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2; SPARC/osteonectin, CWCV, and Kazal-like domains proteoglycan 2
products gene name :
SPOCK2
other gene names :
SPOCK2; SPOCK2; testican-2; KIAA0275; TICN2; UNQ269/PRO306
uniprot entry name :
TICN2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human SPOCK2. No significant cross-reactivity or interference between Human SPOCK2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, saliva and tissue homogenates. Detection Range: 0.78 ng/ml-50 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.195 ng/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ON has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ON present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ON is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ON bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001127906.1
ncbi gb acc num :
NM_001134434.1
ncbi mol weight :
46,779 Da
ncbi pathways :
Hemostasis Pathway 106028!!Platelet Activation, Signaling And Aggregation Pathway 106034!!Platelet Degranulation Pathway 106050!!Response To Elevated Platelet Cytosolic Ca2+ Pathway 106048!!Senescence And Autophagy Pathway 198780!!TGF-beta Receptor Signaling Pathway 198774
ncbi summary :
This gene encodes a protein which binds with glycosaminoglycans to form part of the extracellular matrix. The protein contains thyroglobulin type-1, follistatin-like, and calcium-binding domains, and has glycosaminoglycan attachment sites in the acidic C-terminal region. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
uniprot summary :
Function: May participate in diverse steps of neurogenesis. Binds calcium.