ELISA/assay

Human Neuronal Pentraxin-1, NPTX-1 ELISA Kit

MBS704815

48-Strip-Wells

510 USD

ELISA Kit

Human Neuronal Pentraxin-1, NPTX-1 ELISA Kit

neuronal pentraxin I

Human Neuronal Pentraxin-1 (NPTX-1) ELISA kit; DKFZp686J2446; MGC105123; NP1; ; neuronal pentraxin I

neuronal pentraxin-1; Neuronal pentraxin-1; neuronal pentraxin-1; NP-I; neuronal pentraxin I; Neuronal pentraxin I; NP-I

NPTX1

NPTX1; NPTX1; NP1; NP1; NP-I

NPTX1_HUMAN

Human

432

This assay has high sensitivity and excellent specificity for detection of human NPTX-1. No significant cross-reactivity or interference between human NPTX-1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of human NPTX-1 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NPTX-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPTX-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPTX-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPTX-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

55770878

NP_002513.2

NM_002522.3

Q15818

47,122 Da

NPTX1 is a member of the neuronal pentraxin gene family. Neuronal pentraxin 1 is similar to the rat NP1 gene which encodes a binding protein for the snake venom toxin taipoxin. Human NPTX1 mRNA is exclusively localized to the nervous system. [provided by RefSeq, Jul 2008]

Function: May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses . Cofactor: Binds 2 calcium ions per subunit

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800