product summary
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company name :
MyBioSource
product type :
antibody
product name :
DNASE2 Antibody
catalog :
MBS7048037
quantity :
0.05 mg
price :
160 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, enzyme immunoassay
more info or order :
image
image 1 :
Western Blot. Positive WB detected in: MCF-7 whole cell lysate,PC3 whole cell lysate. All lanes: DNASE2 antibody at 2.7ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40,34 KDa. Observed band size: 40 KDa.
image 2 :
IHC image of MBS7048037 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
product information
catalog number :
MBS7048037
products type :
Antibody
products full name :
DNASE2 Antibody
products short name :
[DNASE2]
products name syn :
[Deoxyribonuclease-2-alpha; Acid DNase; Deoxyribonuclease II alpha; DNase II alpha; Lysosomal DNase II; R31240_2; DNASE2; DNASE2A; DNL2]
other names :
[deoxyribonuclease-2-alpha; Deoxyribonuclease-2-alpha; deoxyribonuclease-2-alpha; deoxyribonuclease 2, lysosomal; Acid DNase; Deoxyribonuclease II alpha; DNase II alpha; Lysosomal DNase II; R31240_2]
products gene name :
[DNASE2]
other gene names :
[DNASE2; DNASE2; DNL; DNL2; DNASE2A; DNASE2A; DNL2; DNase II alpha]
uniprot entry name :
DNS2A_HUMAN
clonality :
Polyclonal
isotype :
IgG
host :
Rabbit
reactivity :
Human
purity :
>95%, Protein G purified
form :
Liquid
storage stability :
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
tested application :
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
app notes :
Recommended Dilution: WB: 1:500-5000, IHC: 1:200-500
image1 heading :
Western Blot (WB)
image2 heading :
Immunohistochemistry (IHC)
other info1 :
Immunogen: Recombinant Human Deoxyribonuclease-2-alpha protein (19-152AA)
other info2 :
Storage Buffer: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. Conjugation: Non-conjugated. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-19271 / sc-19272
products description :
Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
ncbi gi num :
4503349
ncbi acc num :
NP_001366.1
ncbi gb acc num :
NM_001375.2
uniprot acc num :
O00115
ncbi pathways :
Clathrin Derived Vesicle Budding Pathway (1269881); Lysosome Pathway (99052); Lysosome Pathway (96865); Lysosome Vesicle Biogenesis Pathway (1269883); Membrane Trafficking Pathway (1269877); Vesicle-mediated Transport Pathway (1269876); Trans-Golgi Network Vesicle Budding Pathway (1269880)
ncbi summary :
This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008]
uniprot summary :
DNASE2: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. Belongs to the DNase II family. Protein type: Deoxyribonuclease; EC 3.1.22.1. Chromosomal Location of Human Ortholog: 19p13.2. Molecular Function: DNA binding. Biological Process: DNA fragmentation during apoptosis; DNA metabolic process
size1 :
0.05 mg
price1 :
160 USD
size2 :
0.1 mg
price2 :
235
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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