ELISA/assay

Human soluble cluster of differentiation 146, sCD146 ELISA Kit

MBS704803

96 Strip Wells

850 USD

ELISA Kit

Human soluble cluster of differentiation 146, sCD146 ELISA Kit

soluble cluster of differentiation 146, sCD146

Human soluble cluster of differentiation 146; sCD146 ELISA Kit; soluble cluster of differentiation 146; sCD146

sCD146

Human

This assay has high sensitivity and excellent specificity for detection of Human sCD146. No significant cross-reactivity or interference between Human sCD146 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma tissue homogenates. Detection Range: 1.25 ng/ml-80 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.31 ng/ml

Sample Volume: 50-100ul

Introduction: Soluble CD146, a novel endothelial marker, is increased in physiopathological settings linked to endothelial junctional alteration. sCD146 circulates in the plasma of healthy subjects. Modifications of its basal levels could reflect alterations of junctional functions such as vascular permeability. sCD146 involved in monocyte transendothelial migration during inflammation. TNF can increased the release of a soluble form (sCD146) through a metalloproteinase-dependent mechanism. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to sCD146. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for sCD146 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain sCD146, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of sCD146 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

850 USD