catalog number :
MBS704800
products type :
ELISA Kit
products full name :
Human plasmin-antiplasmin complex, PAP ELISA Kit
products short name :
plasmin-antiplasmin complex, PAP
products name syn :
Human plasmin-antiplasmin complex; PAP ELISA Kit; plasmin-antiplasmin complex; PAP
other names :
Pap; Pap; Pap
uniprot entry name :
Q71T77_BPP1
specificity :
This assay has high sensitivity and excellent specificity for detection of human PAP. No significant cross-reactivity or interference between human PAP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates and urine. Assay Type: Sandwich. Detection Range: 0.1 56 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human PAP is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAQ14172.1
ncbi mol weight :
19,219 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells