Human Neuron-specific enolase, NSE ELISA Kit | MyBioSource MBS704796 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Neuron-specific enolase, NSE ELISA Kit

catalog :

MBS704796

quantity :

96 Strip Wells

price :

850 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704796

products type :

ELISA Kit

products full name :

Human Neuron-specific enolase, NSE ELISA Kit

products short name :

enolase 2 (gamma, neuronal)

products name syn :

Human Neuron-specific enolase; NSE ELISA Kit; NSE; 2-phospho-D-glycerate hydrolyase; enolase 2; neural enolase; neuron specific gamma enolase; neurone-specific enolase; enolase 2 (gamma; neuronal)

other names :

gamma-enolase; Gamma-enolase; gamma-enolase; neural enolase; neuron-specific enolase; neurone-specific enolase; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; epididymis secretory protein Li 279; enolase 2 (gamma, neuronal); 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

products gene name :

ENO2

other gene names :

ENO2; ENO2; NSE; HEL-S-279; NSE

uniprot entry name :

ENOG_HUMAN

reactivity :

Human

sequence length :

434

specificity :

This assay has high sensitivity and excellent specificity for detection of Human ENO2. No significant cross-reactivity or interference between Human ENO2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.78 ng/ml-50 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.2 ng/ml

other info2 :

Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: glyconeogenesis and glycometabolism. Protein Biological Process 3: Glycolysis

products description :

Introduction: Neuron Specific Enolase (NSE) is the most abundant form of the glycolytic enolase found in adult neurons and is thought to serve as a growth factor in neurons. Of the three enolase subunits (alpha, beta, and gamma), NSE is a dimer composed of two gamma subunits. NSE is useful in studying neuronal differentiation and is, therefore, a valuable tool for visualizing the entire neuron and endocrine systems. Serum levels of NSE have been associated with such disease states as Alzheimer's, Huntington's Chorea, neuroblastoma, head trauma, neuroendocrine malignancies, and small cell carcinomas of the lung. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NSE. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for NSE to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NSE, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NSE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

5803011

ncbi acc num :

NP_001966.1

ncbi gb acc num :

NM_001975.2

uniprot acc num :

P09104

ncbi mol weight :

47,269 Da

ncbi pathways :

Biosynthesis Of Amino Acids Pathway 790012!!Biosynthesis Of Amino Acids Pathway 795174!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Disease Pathway 530764!!Gluconeogenesis Pathway 106204!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 413342!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 468196!!Glucose Metabolism Pathway 106199!!Glycogen Storage Diseases Pathway 980468

ncbi summary :

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival UniRule annotationHAMAP-Rule:MF_00318. Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotationHAMAP-Rule:MF_00318. Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.

size :

96 Strip Wells

price :

850 USD

more info or order :

MyBioSource product webpage