Rat Stromal cell derived factor 1a, SDF-1a ELISA Kit | MyBioSource MBS704794 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Stromal cell derived factor 1a, SDF-1a ELISA Kit

catalog :

MBS704794

quantity :

48-Strip-Wells

price :

465 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704794

products type :

ELISA Kit

products full name :

Rat Stromal cell derived factor 1a, SDF-1a ELISA Kit

products short name :

Stromal cell derived factor 1a, SDF-1a

products name syn :

Rat Stromal cell derived factor 1a; SDF-1a ELISA Kit; Stromal cell derived factor 1a; SDF-1a

other names :

stromal cell-derived factor 1 isoform alpha; Stromal cell-derived factor 1; stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor; chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF

products gene name :

SDF1a/CXCL12

other gene names :

CXCL12; CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12; SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF

uniprot entry name :

SDF1_HUMAN

reactivity :

Rat

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of rat SDF-1a. No significant cross-reactivity or interference between rat SDF-1a and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 pg/ml-50 pg/ml. Sensitivity: 0.195 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SDF-1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDF-1a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDF-1a is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDF-1a bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

40316924

ncbi acc num :

NP_954637.1

ncbi gb acc num :

NM_199168.3

uniprot acc num :

P48061

ncbi mol weight :

11,004 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); CXCR4-mediated Signaling Events Pathway (137910); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550)

ncbi summary :

This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]

uniprot summary :

Function: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.6 PublicationsManual assertion based on experiment in:Ref.14

size1 :

48-Strip-Wells

price1 :

465 USD

size2 :

96-Strip-Wells

price2 :

655

size3 :

5x96-Strip-Wells

price3 :

2455

size4 :

10x96-Strip-Wells

price4 :

4595

more info or order :

MyBioSource product webpage