catalog number :
MBS704754
products type :
ELISA Kit
products full name :
Mouse interleukin 10, IL-10 ELISA Kit
products short name :
[interleukin 10]
products name syn :
[Mouse interleukin 10; IL-10 ELISA KIT; CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor; interleukin 10]
other names :
[interleukin-10; Interleukin-10; interleukin-10; cytokine synthesis inhibitory factor; interleukin 10; Cytokine synthesis inhibitory factor; CSIF]
products gene name :
[IL10]
other gene names :
[Il10; Il10; CSIF; Il-10; Il-10; IL-10; CSIF]
uniprot entry name :
IL10_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of rabbit IL-1. No significant cross-reactivity or interference between rabbit IL-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034678.1
ncbi gb acc num :
NM_010548.2
ncbi mol weight :
20,641 Da
ncbi pathways :
African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Asthma Pathway (83313); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533)
uniprot summary :
Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells