ELISA/assay

Mouse Bone morphogenetic protein 7, BMP-7 ELISA Kit

MBS704736

48-Strip-Wells

490 USD

ELISA Kit

Mouse Bone morphogenetic protein 7, BMP-7 ELISA Kit

bone morphogenetic protein 7

Mouse Bone morphogenetic protein 7; BMP-7 ELISA Kit; OP-1; osteogenic protein 1; bone morphogenetic protein 7

bone morphogenetic protein 7 preproprotein; Bone morphogenetic protein 7; bone morphogenetic protein 7; osteogenic protein 1; bone morphogenetic protein 7; Osteogenic protein 1; OP-1

BMP7

Bmp7; Bmp7; OP1; Bmp-7; Op1; BMP-7; OP-1

BMP7_MOUSE

Mouse

430

This assay has high sensitivity and excellent specificity for detection of mouse BMP-7. No significant cross-reactivity or interference between mouse BMP-7and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 pg/ml -40 pg/ml. Sensitivity: The minimum detectable dose of mouse BMP-7 is typically less than 0.225 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP-7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP-7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP-7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP-7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

31982487

NP_031583.2

NM_007557.3

P23359

49,199 Da

Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Elastic Fibre Formation Pathway (1001022); Endochondral Ossification Pathway (198336); Extracellular Matrix Organization Pathway (1001015); Hippo Signaling Pathway (749786); Hippo Signaling Pathway (750388); Molecules Associated With Elastic Fibres Pathway (1001023); PodNet: Protein-protein Interactions In The Podocyte Pathway (755428); TGF-beta Signaling Pathway (83261)

This gene encodes a member of a family of secreted factors that participate in developmental signal transduction to stimulate SMAD transcriptional activators. Mutation of this gene results in skeletal, kidney, and other developmental defects. The propeptide is further cleaved into an active chain. [provided by RefSeq, Mar 2013]

Function: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

48-Strip-Wells

490 USD

96-Strip-Wells

690

5x96-Strip-Wells

2560

10x96-Strip-Wells

4790