Human tartrate-resistant acid phosphatase 5b, TRACP-5b ELISA Kit | MyBioSource MBS704711 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human tartrate-resistant acid phosphatase 5b, TRACP-5b ELISA Kit

catalog :

MBS704711

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704711

products type :

ELISA Kit

products full name :

Human tartrate-resistant acid phosphatase 5b, TRACP-5b ELISA Kit

products short name :

acid phosphatase 5, tartrate resistant

products name syn :

Human tartrate-resistant acid phosphatase 5b; TRACP-5b ELISA Kit; MGC117378; TRAP; TrATPase; tartrate resistant acid phosphatase 5; tartrate-resistant acid ATPase; acid phosphatase 5; tartrate resistant

other names :

tartrate-resistant acid phosphatase type 5; Tartrate-resistant acid phosphatase type 5; tartrate-resistant acid phosphatase type 5; TrATPase; tartrate-resistant acid ATPase; acid phosphatase 5, tartrate resistant; Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase

products gene name :

ACP5

other gene names :

ACP5; ACP5; TRAP; SPENCDI; TR-AP; TrATPase

uniprot entry name :

PPA5_HUMAN

reactivity :

Human

sequence length :

325

specificity :

This assay has high sensitivity and excellent specificity for detection of human TRACP-5b. No significant cross-reactivity or interference between human TRACP-5b and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.31 2 mIU/ml -20 mIU/ml. Sensitivity: The minimum detectable dose of human TRACP-5b is typically less than 0.078 mIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TRACP-5b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRACP-5b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRACP-5b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRACP-5b bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

161377451

ncbi acc num :

NP_001104504.1

ncbi gb acc num :

NM_001111034.1

uniprot acc num :

P13686

ncbi mol weight :

36,599 Da

ncbi pathways :

Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009); Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway (906005)

ncbi summary :

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

uniprot summary :

Function: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Catalytic activity: A phosphate monoester + H2O = an alcohol + phosphate. Cofactor: Binds 2 iron ions per subunit.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage