product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse transforming growth factor alpha, TGF-alpha ELISA Kit
catalog :
MBS704708
quantity :
48-Strip-Wells
price :
465 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS704708
products type :
ELISA Kit
products full name :
Mouse transforming growth factor alpha, TGF-alpha ELISA Kit
products short name :
transforming growth factor, alpha
products name syn :
Mouse transforming growth factor alpha; TGF-alpha ELISA Kit; TFGA; transforming growth factor-alpha; transforming growth factor; alpha
other names :
protransforming growth factor alpha preproprotein; Protransforming growth factor alpha; protransforming growth factor alpha; waved 1; transforming growth factor-alpha; transforming growth factor alpha; EGF-like TGF; ETGF; TGF type 1
products gene name :
TGFA
other gene names :
Tgfa; Tgfa; wa1; wa-1; TGF-alpha; ETGF
uniprot entry name :
TGFA_MOUSE
reactivity :
Mouse
sequence length :
159
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse TGF-alpha. No significant cross-reactivity or interference between mouse TGF-alpha and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml -800 pg/ml. Sensitivity: The minimum detectable dose of mouse TGF-alpha is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGF-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
13654266
ncbi acc num :
NP_112476.1
ncbi gb acc num :
NM_031199.3
uniprot acc num :
P48030
ncbi mol weight :
17,018 Da
ncbi pathways :
ErbB Signaling Pathway (83246); ErbB Signaling Pathway (198382); ErbB Signaling Pathway (458); Glioma Pathway (83303); Glioma Pathway (522); Non-small Cell Lung Cancer Pathway (83312); Non-small Cell Lung Cancer Pathway (531); Pancreatic Cancer Pathway (83301); Pancreatic Cancer Pathway (520); Pathways In Cancer (83298)
uniprot summary :
Function: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
size1 :
48-Strip-Wells
price1 :
465 USD
size2 :
96-Strip-Wells
price2 :
655
size3 :
5x96-Strip-Wells
price3 :
2455
size4 :
10x96-Strip-Wells
price4 :
4595
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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