Human Glutathione peroxidase 3, GPX3 ELISA Kit | MyBioSource MBS704704 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Glutathione peroxidase 3, GPX3 ELISA Kit

catalog :

MBS704704

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704704

products type :

ELISA Kit

products full name :

Human Glutathione peroxidase 3, GPX3 ELISA Kit

products short name :

glutathione peroxidase 3

products name syn :

Human Glutathione peroxidase 3 (GPX3) ELISA kit; GPx-P; GSHPx-3; GSHPx-P; extracellular glutathione peroxidase; glutathione peroxidase 3; glutathione peroxidase 3

other names :

glutathione peroxidase 3; Glutathione peroxidase 3; glutathione peroxidase 3; GPx-3; plasma glutathione peroxidase; extracellular glutathione peroxidase; glutathione peroxidase 3 (plasma); Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P

products gene name :

GPX3

other gene names :

GPX3; GPX3; GPx-P; GSHPx-3; GSHPx-P; GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P

uniprot entry name :

GPX3_HUMAN

reactivity :

Human

sequence length :

226

specificity :

This assay has high sensitivity and excellent specificity for detection of human GPX3. No significant cross-reactivity or interference between human GPX3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 uIU/ml -1000 uIU/ml. Sensitivity: The minimum detectable dose of human GPX3 is typically less than 3.9uIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPX3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6006001

ncbi acc num :

NP_002075.2

ncbi gb acc num :

NM_002084.3

uniprot acc num :

P22352

ncbi mol weight :

25,552 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (941400); Folate Metabolism Pathway (198833); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Oxidative Stress Pathway (198916); Selenium Metabolism And Selenoproteins Pathway (198846)

ncbi summary :

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic activity: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage