catalog number :
MBS704702
products type :
ELISA Kit
products full name :
Rabbit Interleukin 1beta, IL-1beta ELISA Kit
products short name :
interleukin 1, beta
products name syn :
Rabbit Interleukin 1beta; IL-1beta ELISA Kit; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; interleukin 1; beta
other names :
interleukin-1 beta; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; interleukin 1 beta; lymphocyte proliferation-potentiating factor; Lymphocyte proliferation-potentiating factor
products gene name :
IL1B
other gene names :
IL1B; IL1B; IL1-BETA; IL-1 beta
uniprot entry name :
IL1B_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rabbit IL-1beta. No significant cross-reactivity or interference between rabbit IL-1beta and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: 1.95 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1beta bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075670.1
ncbi gb acc num :
NM_001082201.1
ncbi mol weight :
30,665 Da
ncbi pathways :
African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Roles Of Anthrax Toxin Pathway (138076); Chagas Disease (American Trypanosomiasis) Pathway (147809)
uniprot summary :
Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
size4 :
10x96-Strip-Wells