ELISA/assay

Human Kallikrein 2, hK 2 ELISA Kit

MBS704696

48-Strip-Wells

565 USD

ELISA Kit

Human Kallikrein 2, hK 2 ELISA Kit

kallikrein-related peptidase 2

Human Kallikrein 2 (hK 2) ELISA Kit; KLK2A2; MGC12201; hK2; glandular kallikrein 2; kallikrein 2; prostatic; kallikrein-related peptidase 2

kallikrein-2 isoform 2 preproprotein; Kallikrein-2; kallikrein-2; tissue kallikrein-2; glandular kallikrein 2; glandular kallikrein-1; kallikrein 2, prostatic; kallikrein-related peptidase 2; Glandular kallikrein-1; hGK-1; Tissue kallikrein-2

KLK2

KLK2; KLK2; hK2; hGK-1; KLK2A2; hGK-1

KLK2_HUMAN

Human

223

This assay has high sensitivity and excellent specificity for detection of human hK 2. No significant cross-reactivity or interference between human hK 2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: 15.6 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for hK 2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any hK 2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for hK 2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of hK 2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

50363237

NP_001002231.1

NM_001002231.2

P20151

28,671 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Coregulation Of Androgen Receptor Activity Pathway (138085); Degradation Of The Extracellular Matrix Pathway (576263); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Extracellular Matrix Organization Pathway (576262); Metabolism Of Proteins Pathway (106230); Prostate Cancer Pathway (755440); Regulation Of Androgen Receptor Activity Pathway (138027); Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway (105908)

This gene encodes a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein encoded by this gene is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. This protein is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Jan 2012]

Function: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Catalytic activity: Preferential cleavage of Arg- -Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met- -Xaa or Leu- -Xaa.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150