ELISA/assay

Human Soluble Interleukin-7 Receptor, sIL-7R ELISA Kit

MBS704684

96 Strip Wells

850 USD

ELISA Kit

Human Soluble Interleukin-7 Receptor, sIL-7R ELISA Kit

interleukin 7 receptor

Human Soluble Interleukin-7 Receptor; sIL-7R ELISA Kit; CD127; CDW127; IL-7R-alpha; IL7RA; ILRA; CD127 antigen; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor

interleukin-7 receptor subunit alpha; Interleukin-7 receptor subunit alpha; interleukin-7 receptor subunit alpha; IL-7RA; CD127 antigen; IL-7R subunit alpha; IL-7 receptor subunit alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; CDw127; CD_antigen: CD127

IL7R

IL7R; IL7R; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA

IL7RA_HUMAN

Human

459

This assay has high sensitivity and excellent specificity for detection of Human IL7R. No significant cross-reactivity or interference between Human IL7R and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and tissue homogenates. Detection Range: 0.78 ng/ml -50 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.195 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to sIL-7R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for sIL-7R and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain sIL-7R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of sIL-7R in the samples is then determined by comparing the O.D. of the samples to the standard curve.

28610151

NP_002176.2

NM_002185.3

P16871

51,581 Da

Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!FoxO Signaling Pathway 921162!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!IL-7 Signaling Pathway 198857!!Immune System Pathway 106386!!Interleukin-7 Signaling Pathway 530762!!Jak-STAT Signaling Pathway 83077

The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2014]

Function: Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

96 Strip Wells

850 USD