ELISA/assay

Rat Interleukin 23, IL-23 ELISA Kit

MBS704680

96 Strip Wells

575 USD

ELISA Kit

Rat Interleukin 23, IL-23 ELISA Kit

Interleukin 23, IL-23

Rat Interleukin 23; IL-23 ELISA Kit; Interleukin 23; IL-23

IL-23

Rat

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 3.12 pg/ml-200 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.78 pg/ml

Sample Volume: 50-100ul

Introduction: Interleukin-23 (IL-23) is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. In conjunction with IL-6 and TGF-beta1, IL-23 stimulates naive CD4+ T cells to differentiate into a novel subset of cells called Th17 cells, which are distinct from the classical Th1 and Th2 cells. Th17 cells produce IL-17, a proinflammatory cytokine that enhances T cell priming and stimulates the production of proinflammatory molecules such as IL-1, IL-6, TNF-alpha, NOS-2, and chemokines resulting in inflammation. Knockout mice deficient in either p40 or p19, or in either subunit of the IL-23 receptor (IL-23R and IL12R-beta1) develop less severe symptoms of multiple sclerosis and inflammatory bowel disease highlighting the importance of IL-23 in the inflammatory pathway. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-23. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-23 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-23, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-23 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

575 USD