ELISA/assay

Rat Insulin-like Growth Factors 1 Receptor, IGF-1R ELISA Kit

MBS704674

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Rat Insulin-like Growth Factors 1 Receptor, IGF-1R ELISA Kit

[insulin-like growth factor 1 receptor]

[Rat Insulin-like Growth Factors 1 Receptor; IGF-1R ELISA Kit; CD221; IGFIR; IGFR; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor 1 receptor]

[insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor; insulin-like growth factor 1 receptor; IGF-I receptor; insulin-like growth factor I receptor; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD_antigen: CD221Cleaved into the following 2 chains:Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain]

[IGF1R]

[Igf1r; Igf1r; JTK13; IGFIRC; IGF-I receptor]

IGF1R_RAT

Rat

1370

This assay has high sensitivity and excellent specificity for detection of rat IGF-1R. No significant cross-reactivity or interference between rat IGF-1R and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: Typically less than 0.8 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF-1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF-1R is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF-1R bound in the initial step. The color development is stopped and the intensity of the color is measured.

16258823

NP_434694.1

NM_052807.2

P24062

155,396 Da

AMPK Signaling Pathway (989832); AMPK Signaling Pathway (992181); Adherens Junction Pathway (83462); Adherens Junction Pathway (481); Apoptosis Pathway (219785); Endochondral Ossification Pathway (219771); Endocytosis Pathway (102267); Endocytosis Pathway (102181); Focal Adhesion Pathway (83459); Focal Adhesion Pathway (478)

receptor for Igf-1; involved in induction of cell cycle progression and survival in many cell types [RGD, Feb 2006]

Function: Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R When present in a hybrid receptor with INSR, binds IGF1 1 PublicationManual assertion based on experiment in:Ref.4. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Enzyme regulation: Activated by autophosphorylation at Tyr-1162, Tyr-1166 and Tyr-1167 on the kinase activation loop; phosphorylation at all three tyrosine residues is required for optimal kinase activity. Inhibited by MSC1609119A-1, BMS-754807, PQIP, benzimidazole pyridinone, isoquinolinedione, bis-azaindole, 3-cyanoquinoline, 2,4-bis-arylamino-1,3-pyrimidine, pyrrolopyrimidine, pyrrole-5-carboxaldehyde, picropodophyllin (PPP), tyrphostin derivatives. While most inhibitors bind to the ATP binding pocket, MSC1609119A-1 functions as allosteric inhibitor and binds close to the DFG motif and the activation loop Dephosphorylated by PTPN1

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

550

96-Strip-Wells

795

5x96-Strip-Wells

2890

10x96-Strip-Wells

5465