Mouse Interleukin 18, IL-18 ELISA Kit | MyBioSource MBS704673 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Interleukin 18, IL-18 ELISA Kit

catalog :

MBS704673

quantity :

96 Strip Wells

price :

760 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704673

products type :

ELISA Kit

products full name :

Mouse Interleukin 18, IL-18 ELISA Kit

products short name :

interleukin 18 (interferon-gamma-inducing factor)

products name syn :

Mouse Interleukin 18; IL-18 ELISA Kit; IGIF; IL-18; IL-1g; IL1F4; MGC12320; IL-1 gamma; interferon-gamma-inducing factor; interleukin 18; interleukin-1 gamma; interleukin-18; interleukin 18 (interferon-gamma-inducing factor)

other names :

interleukin-18; Interleukin-18; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; interleukin 18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma

products gene name :

IL18

other gene names :

Il18; Il18; Igif; Il-18; Igif; IL-18; IFN-gamma-inducing factor; IL-1 gamma

uniprot entry name :

IL18_MOUSE

reactivity :

Mouse

sequence length :

192

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse IL18. No significant cross-reactivity or interference between Mouse IL18 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 1.56 pg/ml-100 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.39 pg/ml

other info2 :

Sample Volume: 50-100ul

products description :

Introduction: Interleukin-18 (IL-18), a recently described member of the IL-1 cytokine superfamily, is now recognized as an important regulator of innate and acquired immune responses. IL-18 is expressed at sites of chronic inflammation, in autoimmune diseases, in a variety of cancers, and in the context of numerous infectious diseases. IL-18 works together with IL-12 to induce cell-mediated immunity followipg infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-gamma (IFN-gamma) or type II interferon that plays an important role in activatipg the macrophages or other cells. Apart from its physiological role, IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders. The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 bindipg protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-18. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody prepahumanion specific for IL-18 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substhumane solution is added to each well. Only those wells that contain IL-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a chapge in color. The enzyme-substhumane reaction is terminated by the addition of a sulphuric acid solution and the color chapge is measured spectrophotometrically at a wavelepgth of 450 nm +/- 2 nm. The concenthumanion of IL-18 in the samples is then determined by comparipg the O.D. of the samples to the standard curve.

ncbi gi num :

6680413

ncbi acc num :

NP_032386.1

ncbi gb acc num :

NM_008360.1

uniprot acc num :

P70380

ncbi mol weight :

22,135 Da

ncbi pathways :

African Trypanosomiasis Pathway 194387!!African Trypanosomiasis Pathway 194323!!Cytokine Signaling In Immune System Pathway 970814!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytosolic DNA-sensing Pathway 125146!!Cytosolic DNA-sensing Pathway 124833!!Hypertrophy Model Pathway 198326!!Immune System Pathway 971216!!Inflammatory Bowel Disease (IBD) Pathway 842778

uniprot summary :

Function: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

size :

96 Strip Wells

price :

760 USD

more info or order :

MyBioSource product webpage