catalog number :
MBS704670
products type :
ELISA Kit
products full name :
Chicken Heat Shock Protein 90, HSP-90 ELISA Kit
products short name :
heat shock protein 90kDa alpha (cytosolic) , class A member 1
products name syn :
Chicken Heat Shock Protein 90; HSP-90 ELISA Kit; FLJ31884; HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp89; Hsp90; LAP2; heat shock 90kD protein 1; alpha; heat shock 90kD protein 1; alpha-like 4; heat shock 90kD protein; a; heat shock protein 90kDa alpha (cytosolic); class A member 1
other names :
heat shock protein HSP 90-alpha; Heat shock protein HSP 90-alpha; heat shock protein HSP 90-alpha; hypothetical protein; heat shock protein HSP90 alpha; heat shock protein 90kDa alpha (cytosolic), class A member 1
products gene name :
HSP90AA1
other gene names :
HSP90AA1; HSP90AA1; HSPCA
uniprot entry name :
HS90A_CHICK
specificity :
This assay has high sensitivity and excellent specificity for detection of Chicken HSP90AA1. No significant cross-reactivity or interference between Chicken HSP90AA1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml-100 ng/ml. Sensitivity: 0.39 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Neurobiology. Protein Biological Process 3: Stress response
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSP-90 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP-90 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP-90 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP-90 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001103255.1
ncbi gb acc num :
NM_001109785.1
ncbi mol weight :
84,059 Da
ncbi pathways :
NOD-like Receptor Signaling Pathway (122198); NOD-like Receptor Signaling Pathway (122131); Progesterone-mediated Oocyte Maturation Pathway (119322); Progesterone-mediated Oocyte Maturation Pathway (503); Protein Processing In Endoplasmic Reticulum Pathway (167335); Protein Processing In Endoplasmic Reticulum Pathway (167190)
uniprot summary :
Function: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function UniRule annotationHAMAP-Rule:MF_00505
size4 :
10x96-Strip-Wells