Human angiotensin II receptor 1, ANGIIR-1 ELISA Kit | MyBioSource MBS704669 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human angiotensin II receptor 1, ANGIIR-1 ELISA Kit

catalog :

MBS704669

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704669

products type :

ELISA Kit

products full name :

Human angiotensin II receptor 1, ANGIIR-1 ELISA Kit

products short name :

angiotensin II receptor, type 1

products name syn :

Human angiotensin II receptor 1; ANGIIR-1 ELISA Kit; AG2S; AGTR1A; AGTR1B; AT1; AT1B; AT1R; AT2R1; AT2R1A; AT2R1B; HAT1R; angiotensin receptor 1; angiotensin receptor 1B; type-1B angiotensin II receptor; angiotensin II receptor; type 1

other names :

type-1 angiotensin II receptor isoform 1; Type-1 angiotensin II receptor; type-1 angiotensin II receptor; type-1B angiotensin II receptor; angiotensin II receptor, type 1; AT1AR; AT1BR; Angiotensin II type-1 receptor; AT1

products gene name :

AGTR1

other gene names :

AGTR1; AGTR1; AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1B; AGTR1A; AGTR1B; AT2R1; AT2R1B; AT1

uniprot entry name :

AGTR1_HUMAN

reactivity :

Human

sequence length :

359

specificity :

This assay has high sensitivity and excellent specificity for detection of human ANG II R-1. No significant cross-reactivity or interference between human ANG II R-1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml-3000 pg/ml. Sensitivity: The minimum detectable dose of human ANG II R-1 is typically less than 11.72 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANGII R-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANGII R-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANGII R-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANGII R-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4501997

ncbi acc num :

NP_000676.1

ncbi gb acc num :

NM_000685.4

uniprot acc num :

P30556

ncbi mol weight :

41,061 Da

ncbi pathways :

ACE Inhibitor Pathway (198763); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Arf6 Signaling Events Pathway (138034); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548)

ncbi summary :

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012]

uniprot summary :

Function: Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage