human snail homolog 2, SNAI2 ELISA Kit | MyBioSource MBS704660 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

human snail homolog 2, SNAI2 ELISA Kit

catalog :

MBS704660

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704660

products type :

ELISA Kit

products full name :

human snail homolog 2, SNAI2 ELISA Kit

products short name :

snail homolog 2 (Drosophila)

products name syn :

human snail homolog 2; SNAI2 ELISA Kit; MGC10182; SLUG; SLUGH1; WS2D; OTTHUMP00000195093; neural crest transcription factor SLUG; slug (chicken homolog); zinc finger protein; snail 2; snail homolog 2 (Drosophila)

other names :

zinc finger protein SNAI2; Zinc finger protein SNAI2; zinc finger protein SNAI2; snail homolog 2; protein snail homolog 2; neural crest transcription factor SLUG; slug (chicken homolog), zinc finger protein; snail family zinc finger 2; Neural crest transcription factor Slug; Protein snail homolog 2

products gene name :

SNAI2

other gene names :

SNAI2; SNAI2; SLUG; WS2D; SLUGH1; SNAIL2; SLUG; SLUGH

uniprot entry name :

SNAI2_HUMAN

reactivity :

Human

sequence length :

268

specificity :

This assay has high sensitivity and excellent specificity for detection of human SNAI2. No significant cross-reactivity or interference between human SNAI2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human SNAI2 is typically less than 7.81 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SNAI2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SNAI2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SNAI2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SNAI2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

11276067

ncbi acc num :

NP_003059.1

ncbi gb acc num :

NM_003068.4

uniprot acc num :

O43623

ncbi mol weight :

29,986 Da

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Direct P53 Effectors Pathway (137939); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Neural Crest Differentiation Pathway (672460); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); Signaling Events Mediated By Stem Cell Factor Receptor (c-Kit) Pathway (138068)

ncbi summary :

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.6 PublicationsManual assertion based on experiment in:Ref.1

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage