catalog number :
MBS704640
products type :
ELISA Kit
products full name :
Mouse proteoglycan 4, PRG4 ELISA Kit
products short name :
proteoglycan 4
products name syn :
Mouse proteoglycan 4 (PRG4) ELISA Kit; GS1-174L6.2; CACP; FLJ32635; HAPO; JCAP; MSF; SZP; bG174L6.2; OTTHUMP00000033580; articular superficial zone protein; bG174L6.2 (MSF: megakaryocyte stimulating factor) ; lubricin; megakaryocyte stimulat; proteoglycan 4
other names :
proteoglycan 4 isoform 2; Proteoglycan 4; proteoglycan 4; proteoglycan 3; superficial zone proteoglycan; megakaryocyte-stimulating factor; proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein); Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycanCleaved into the following chain:Proteoglycan 4 C-terminal part
products gene name :
PRG4
other gene names :
Prg4; Prg4; MSF; SZP; CACP; JCAP; DOL54; lubricin; Msf; Szp
uniprot entry name :
PRG4_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse PRG4. No significant cross-reactivity or interference between Mouse PRG4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRG4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001103616.1
ncbi gb acc num :
NM_001110146.1
ncbi mol weight :
115,996 Da
uniprot summary :
Function: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.1 PublicationManual assertion based on experiment in:Ref.3
size4 :
10x96-Strip-Wells