Human Mast Cell Chymase ELISA Kit | MyBioSource MBS704628 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Mast Cell Chymase ELISA Kit

catalog :

MBS704628

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704628

products type :

ELISA Kit

products full name :

Human Mast Cell Chymase ELISA Kit

products short name :

chymase 1, mast cell

products name syn :

Human Mast Cell Chymase ELISA Kit; CYH; MCT1; MGC119890; MGC119891; chymase; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase; heart; chymase; mast cell; mast cell protease I; chymase 1; mast cell

other names :

chymase preproprotein; Chymase; chymase; alpha-chymase; chymase, heart; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; Alpha-chymase; Mast cell protease I

products gene name :

CMA1

other gene names :

CMA1; CMA1; CYH; MCT1; chymase; CYH; CYM

uniprot entry name :

CMA1_HUMAN

reactivity :

Human

sequence length :

247

specificity :

This assay has high sensitivity and excellent specificity for detection of human mast cell chymase. No significant cross-reactivity or interference between human mast cell chymase and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human mast cell chymase is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for mast cell chymase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any mast cell chymase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for mast cell chymase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mast cell chymase bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4502907

ncbi acc num :

NP_001827.1

ncbi gb acc num :

NM_001836.3

uniprot acc num :

P23946

ncbi mol weight :

27,325 Da

ncbi pathways :

ACE Inhibitor Pathway (198763); Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486); Signal Transduction Pathway (477114)

ncbi summary :

This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Catalytic activity: Preferential cleavage: Phe- -Xaa > Tyr- -Xaa > Trp- -Xaa > Leu- -Xaa.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage