ELISA/assay

Rat Kisspeptin 1, KISS1 ELISA Kit

MBS704611

96 Strip Wells

850 USD

ELISA Kit

Rat Kisspeptin 1, KISS1 ELISA Kit

KiSS-1 metastasis-suppressor

Rat Kisspeptin 1 (KISS1) ELISA Kit; KiSS-1; METASTIN; MGC39258; kisspeptin; malignant melanoma metastasis-suppressor; KiSS-1 metastasis-suppressor

metastasis-suppressor KiSS-1; Metastasis-suppressor KiSS-1; metastasis-suppressor KiSS-1; metastin; kisspeptin-1; MLL septin-like fusion; Kiss1 variant E1a-E2b-E3; Kiss1 variant E1b-E2a-E3; Kiss1 variant E1b-E2b-E3; KiSS-1 metastasis-suppressor; Kisspeptin-1Cleaved into the following 2 chains:Metastin; Kisspeptin-10Alternative name(s):Metastin45-54

KISS1

Kiss1; Kiss1; Eseptin

KISS1_RAT

Rat

130

This assay has high sensitivity and excellent specificity for detection of Rat KISS1. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KISS1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for KISS1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain KISS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of KISS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

32140194

NP_859043.1

NM_181692.1

Q7TSB7

14,188 Da

Class A/1 (Rhodopsin-like Receptors) Pathway 1010164!!G Alpha (q) Signalling Events Pathway 1010197!!GPCR Downstream Signaling Pathway 1010193!!GPCR Ligand Binding Pathway 1010163!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 1010213!!Peptide Ligand-binding Receptors Pathway 1010165!!Signal Transduction Pathway 1010015!!Signaling By GPCR Pathway 1010162

ligand for the G-protein-coupled receptor GPR54; may be involved in autonomic and sensory neural signaling [RGD, Feb 2006]

Function: Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals.3 PublicationsManual assertion based on experiment in:Ref.2

96 Strip Wells

850 USD