ELISA/assay

Rat kininogen, KNG ELISA Kit

MBS704587

96 Strip Wells

895 USD

ELISA Kit

Rat kininogen, KNG ELISA Kit

kininogen 1

Rat kininogen (KNG) ELISA Kit; BDK; KNG; alpha-2-thiol proteinase inhibitor; bradykinin; kininogen 1

kininogen-1 isoform 1; Kininogen-1; kininogen-1; K-kininogen; kininogen 2

KNG1

Kng2; Kng1; Kng1; Kngk; KINKG; KINKH; Kng

KNG1_RAT

Rat

639

This assay has high sensitivity and excellent specificity for detection of Rat KNG1. No significant cross-reactivity or interference between Rat KNG1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 1.6 ng/ml-100 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.4 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Blood Coagulation. Protein Biological Process 3: Blood coagulation

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KNG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for KNG and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain KNG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of KNG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

7549773

NP_036873.1

NM_012741.1

P08934

70,933 Da

Complement And Coagulation Cascades Pathway 83465!!Complement And Coagulation Cascades Pathway 484

precursor protein of kinin which is found in plasma; cysteine protease inhibitor and a major acute phase reactant [RGD, Feb 2006]

Function: 1 Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

96 Strip Wells

895 USD