Human integrin-linked kinase 1, ILK-1 ELISA Kit | MyBioSource MBS704581 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human integrin-linked kinase 1, ILK-1 ELISA Kit

catalog :

MBS704581

quantity :

96 Strip Wells

price :

760 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704581

products type :

ELISA Kit

products full name :

Human integrin-linked kinase 1, ILK-1 ELISA Kit

products short name :

integrin-linked kinase

products name syn :

Human integrin-linked kinase 1; ILK-1 ELISA Kit; DKFZp686F1765; P59; ; integrin-linked kinase

other names :

integrin-linked protein kinase isoform 1; Integrin-linked protein kinase; integrin-linked protein kinase; integrin-linked kinase-2; epididymis secretory protein Li 28; 59 kDa serine/threonine-protein kinase; integrin-linked kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK

products gene name :

ILK

other gene names :

ILK; ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28; ILK1; ILK2

uniprot entry name :

ILK_HUMAN

reactivity :

Human

sequence length :

452

specificity :

This assay has high sensitivity and excellent specificity for detection of Human ILK. No significant cross-reactivity or interference between Human ILK and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm

other info2 :

Sample Volume: 50-100ul

products description :

Introduction: Integrin-linked kinase (ILK) is a 59kDa protein originally identified while conducting a yeast-two hybrid screen with integrin beta1 as the bait protein (Hannigan et al., 1996). Since its discovery, ILK has been associated with multiple cellular functions including cell migration, cell proliferation, cell-adhesions, signal transduction. ILK1 is a member of the serine/threonine kinases, has been demonstrated to be associated with numerous biological and pathological processes. However, the clinical and functional significance of ILK1 expression has not been characterized previously in human astrocytoma. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to ILK-1 has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated ILK-1 and incubated. A competitive inhibition reaction is launched between ILK-1 (Standards or samples) and HRP-conjugated ILK-1 with the pre-coated antibody specific for ILK-1. The more amount of ILK-1 in samples, the less antibody bound by HRP-conjugated ILK-1. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of ILK-1 in the sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

62420873

ncbi acc num :

NP_001014794.1

ncbi gb acc num :

NM_001014794.2

uniprot acc num :

Q13418

ncbi mol weight :

51,419 Da

ncbi pathways :

Bacterial Invasion Of Epithelial Cells Pathway 149807!!Bacterial Invasion Of Epithelial Cells Pathway 148661!!Cell Junction Organization Pathway 160966!!Cell-Cell Communication Pathway 477132!!Cell-extracellular Matrix Interactions Pathway 160967!!Endometrial Cancer Pathway 83109!!Endometrial Cancer Pathway 521!!Eukaryotic Transcription Initiation Pathway 198917!!Focal Adhesion Pathway 198795!!Focal Adhesion Pathway 83067

ncbi summary :

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

uniprot summary :

Function: Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Enzyme regulation: Stimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK.

size :

96 Strip Wells

price :

760 USD

more info or order :

MyBioSource product webpage