Human A Disintegrin And Metalloprotease 12, ADAM12 ELISA Kit | MyBioSource MBS704578 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human A Disintegrin And Metalloprotease 12, ADAM12 ELISA Kit

catalog :

MBS704578

quantity :

48-Strip-Wells

price :

590 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704578

products type :

ELISA Kit

products full name :

Human A Disintegrin And Metalloprotease 12, ADAM12 ELISA Kit

products short name :

ADAM metallopeptidase domain 12

products name syn :

Human A Disintegrin And Metalloprotease 12; ADAM12 ELISA Kit; RP11-295J3.5; MCMP; MCMPMltna; MLTN; MLTNA; a disintegrin and metalloproteinase domain 12 (meltrin alpha) ; meltrin alpha; ADAM metallopeptidase domain 12

other names :

disintegrin and metalloproteinase domain-containing protein 12 isoform 4 preproprotein; Disintegrin and metalloproteinase domain-containing protein 12; disintegrin and metalloproteinase domain-containing protein 12; meltrin-alpha; metalloprotease-disintegrin 12 transmembrane; ADAM metallopeptidase domain 12; Meltrin-alpha

products gene name :

ADAM12

other gene names :

ADAM12; ADAM12; MCMP; MLTN; CAR10; MLTNA; MCMPMltna; ADAM12-OT1; MLTN; UNQ346/PRO545; ADAM 12

uniprot entry name :

ADA12_HUMAN

reactivity :

Human

sequence length :

737

specificity :

This assay has high sensitivity and excellent specificity for detection of Human ADAM12. No significant cross-reactivity or interference between Human ADAM12 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine, cell culture supernates, tissue homogenates, Cell lysates. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.8 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADAM12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ADAM12 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADAM12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADAM12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

572882491

ncbi acc num :

NP_001275903.1

ncbi gb acc num :

NM_001288974.1

uniprot acc num :

O43184

ncbi mol weight :

99,542 Da

ncbi pathways :

Disease Pathway (530764); Notch Signaling Pathway (169345); Signal Transduction Pathway (477114); Signaling By EGFR Pathway (106337); Signaling By EGFR In Cancer Pathway (530768); Syndecan-4-mediated Signaling Events Pathway (138064)

ncbi summary :

This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]

uniprot summary :

Function: Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors . Cofactor: Binds 1 zinc ion per subunit.

size1 :

48-Strip-Wells

price1 :

590 USD

size2 :

96-Strip-Wells

price2 :

850

size3 :

5x96-Strip-Wells

price3 :

2880

size4 :

10x96-Strip-Wells

price4 :

5440

more info or order :

MyBioSource product webpage