ELISA/assay

Rat Matrix Metalloproteinase 14, MMP-14 ELISA Kit

MBS704577

48-Strip-Wells

565 USD

ELISA Kit

Rat Matrix Metalloproteinase 14, MMP-14 ELISA Kit

matrix metallopeptidase 14 (membrane-inserted)

Rat Matrix Metalloproteinase 14; MMP-14 ELISA Kit; MMP-X1; MT1-MMP; MTMMP1; matrix metalloproteinase 14; matrix metalloproteinase 14 (membrane-inserted) ; membrane type 1 metalloprotease; membrane-type matrix metalloproteinase 1; membrane-type-1 matrix m; matrix metallopeptidase 14 (membrane-inserted)

matrix metalloproteinase-14; Matrix metalloproteinase-14; matrix metalloproteinase-14; MMP-14; MT1MMP; MTMMP1; MT1-MMP; MT-MMP 1; matrix metalloproteinase 14; membrane type 1 metalloproteinase; membrane-type matrix metalloproteinase 1; membrane-type-1 matrix metalloproteinase; matrix metallopeptidase 1 (interstitial collagenase); Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP

MMP14

MMP14; MMP14; MMP1; MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP

MMP14_RABIT

Rat

582

This assay has high sensitivity and excellent specificity for detection of rat MMP-14. No significant cross-reactivity or interference between rat MMP-14 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml -3000 pg/ml. Sensitivity: 11.75 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-14 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

130486970

NP_001076262.1

NM_001082793.1

Q95220

65,963 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); GnRH Signaling Pathway (83091); GnRH Signaling Pathway (502); HIF-2-alpha Transcription Factor Network Pathway (137956); Matrix Metalloproteinases Pathway (198900); Senescence And Autophagy Pathway (198780); Signaling Events Mediated By Focal Adhesion Kinase Pathway (169348)

Function: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface May be involved in actin cytoskeleton reorganization by cleaving PTK7 Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues . Catalytic activity: Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn- -Leu-38. Other bonds hydrolyzed include 35-Gly- -Ile-36 in the propeptide of collagenase 3, and 341-Asn- -Phe-342, 441-Asp- -Leu-442 and 354-Gln- -Thr-355 in the aggrecan interglobular domain. Cofactor: Binds 1 zinc ion per subunit

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150