Mouse tissue inhibitors of metalloproteinase 1, TIMP-1 ELISA Kit | MyBioSource MBS704573 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse tissue inhibitors of metalloproteinase 1, TIMP-1 ELISA Kit

catalog :

MBS704573

quantity :

96-Strip-Wells

price :

680 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704573

products type :

ELISA Kit

products full name :

Mouse tissue inhibitors of metalloproteinase 1, TIMP-1 ELISA Kit

products short name :

TIMP metallopeptidase inhibitor 1

products name syn :

Mouse tissue inhibitors of metalloproteinase 1; TIMP-1 ELISA Kit; RP1-230G1.3; CLGI; EPA; EPO; FLJ90373; HCI; TIMP; erythroid potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinase 1; Mouse tissue inhibitors of metalloproteinase 1; TIMP-1

other names :

metalloproteinase inhibitor 1 isoform a; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; TPA-induced protein; erythroid-potentiating activity; collagenase inhibitor 16C8 fibroblast; tissue inhibitor of metalloproteinase-1; tissue inhibitor of metalloproteinases 1; tissue inhibitor of metalloproteinase 1; Collagenase inhibitor 16C8 fibroblast; Erythroid-potentiating activity; EPA; TPA-S1; TPA-induced protein; Tissue inhibitor of metalloproteinases 1; TIMP-1

products gene name :

TIMP1

other gene names :

Timp1; Timp1; EPA; Clgi; Timp; TIMP-1; TPA-S1; Timp; Timp-1; EPA; TIMP-1

uniprot entry name :

TIMP1_MOUSE

reactivity :

Mouse

sequence length :

205

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse TIMP-1. No significant cross-reactivity or interference between mouse TIMP-1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and cell culture supernates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: The minimum detectable dose of mouse TIMP-1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TIMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

113205063

ncbi acc num :

NP_001037849.1

ncbi gb acc num :

NM_001044384.1

uniprot acc num :

P12032

ncbi mol weight :

22,628 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1001029); Degradation Of The Extracellular Matrix Pathway (1001028); Extracellular Matrix Organization Pathway (1001015); HIF-1 Signaling Pathway (695223); Hemostasis Pathway (1001295); Matrix Metalloproteinases Pathway (198370); Platelet Activation, Signaling And Aggregation Pathway (1001305); Platelet Degranulation Pathway (1001317); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1001315)

uniprot summary :

Function: Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.1 PublicationManual assertion based on experiment in:Ref.7

size1 :

96-Strip-Wells

price1 :

680 USD

size2 :

5x96-Strip-Wells

price2 :

2140

size3 :

10x96-Strip-Wells

price3 :

3990

more info or order :

MyBioSource product webpage