ELISA/assay

Mouse adiponectin, ADP ELISA Kit

MBS704558

96 Strip Wells

655 USD

ELISA Kit

Mouse adiponectin, ADP ELISA Kit

adiponectin, C1Q and collagen domain containing

Mouse adiponectin; ADP ELISA Kit; ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28; adiponectin; OTTHUMP00000210047; OTTHUMP00000210048; adipocyte; C1Q and collagen domain containing; adipocyte; C1Q and collagen domain-containing; adipo; Mouse adiponectin; ADP

adiponectin; Adiponectin; adiponectin; adipocyte-specific protein AdipoQ; adipocyte complement related protein; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; adiponectin, C1Q and collagen domain containing; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ

ADP

Adipoq; Adipoq; APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30; Acdc; Acrp30; Apm1; ACRP30

ADIPO_MOUSE

Mouse

247

This assay has high sensitivity and excellent specificity for detection of Mouse ADIPOQ. No significant cross-reactivity or interference between Mouse ADIPOQ and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Detection Range: 0.156 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.039 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Cardiovascular

Introduction: Adiponectin (also referred to as Acrp30, apM1) is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid catabolism. Adiponectin is exclusively secreted from adipose tissue into the bloodstream and is very abundant in plasma relative to many hormones. Levels of the hormone are inversely correlated with body fat percentage in adults, while the association in infants and young children is more unclear. The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Adiponectin is a 244-amino-acid-long polypeptide. There are four distinct regions of adiponectin. The first is a short signal squence that targets the hormone for secretion outside the cell; next is a short region that varies between species; the third is a 65-amino acid region with similarity to collagenous proteins; the last is a globular domain. Overall this gene shows similarity to the complement 1Q factors. However, when the 3-dimensional structure of the globular region was determined, a striking similarity to TNFalpha was observed, despite unrelated protein sequences. Adiponectin is secreted into the bloodsteam where it accounts for approximately 0.01% of all plasma protein at around 5-10 ug/mL. Plasma concentrations reveal a sexual dimorphism, with females having higher levels than males. Levels of adiponectin are reduced in diabetics compared to non-diabetics. Weight reduction significantly increases circulating levels. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ADP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

87252711

NP_033735.3

NM_009605.4

Q60994

26,809 Da

AMPK Signaling Pathway 989651!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83290!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198299!!Developmental Biology Pathway 970813!!Leptin And Adiponectin Pathway 198344!!Non-alcoholic Fatty Liver Disease (NAFLD) Pathway 862220!!Non-alcoholic Fatty Liver Disease (NAFLD) Pathway 862314!!PPAR (Peroxisome Proliferator-activated Receptor) Signaling Pathway 522983

Function: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.5 PublicationsManual assertion based on experiment in:Ref.11

96 Strip Wells

655 USD