ELISA/assay

Canine Thyroid Stimulating Hormone, TSH ELISA Kit

MBS704551

96 Strip Wells

645 USD

ELISA Kit

Canine Thyroid Stimulating Hormone, TSH ELISA Kit

Thyroid Stimulating Hormone, TSH

Canine Thyroid Stimulating Hormone; TSH ELISA Kit; Thyroid Stimulating Hormone; TSH

Tsh; Temperature-sensitive hemagglutinin tsh autotransporter; Autotransporter tshCleaved into the following 2 chains:Temperature-sensitive hemagglutinin tsh; Temperature-sensitive hemagglutinin tsh translocator

TSH

tsh

TSH_ECOLX

Dog

1377

This assay has high sensitivity and excellent specificity for detection of Dog TSH. No significant cross-reactivity or interference between Dog TSH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and tissue homogenates. Detection Range: 0.625 uIU/ml-10 uIU/ml. Detection Wavelength: 450 nm. Sensitivity: 0.312 uIU/ml

Sample Volume: 50-100ul

Introduction: Thyroid-stimulating hormone (also known as TSH or thyrotropin) is a peptide hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroid gland. TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4) and triiodothyronine (T3).TSH production is controlled by thyrotropin-releasing hormone (TRH), which is manufactured in the hypothalamus and transported to the anterior pituitary gland via the superior hypophyseal artery, where it increases TSH production and release. Somatostatin is also produced by the hypothalamus, and has an opposite effect on the pituitary production of TSH, decreasing or inhibiting its release. The level of thyroid hormones (T3 and T4) in the blood have an effect on the pituitary release of TSH; when the levels of T3 and T4 are low, the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased. This effect creates a regulatory negative feedback loop. Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. An antibody specific for TSH has been pre-coated onto a microplate. Add TSH (Standards or samples) to the well, and then add Biotin conjugated TSH. A competitive inhibition reaction is launched between TSH (Standards or samples) and Biotin conjugated TSH with the TSH antibody. Then add the HRP-avidin to each well. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of TSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

469236

AAA24698.1

Q47692

148,227 Da

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

Function: Contributes to the development of lesions and deposition of fibrin in the avian air sacs. It can act both as an adhesin and as a serine protease. Agglutinates erythrocytes while in contact with the extracellular surface of the bacterial cells. Can adhere to purified hemoglobin and bind with great efficiency to extracellular matrix proteins. Cleaves casein and exhibits mucinolytic activity.4 PublicationsManual assertion based on experiment in:Ref.1

96 Strip Wells

645 USD