catalog number :
MBS704529
products type :
ELISA Kit
products full name :
Human brain natriuretic peptide, BNP ELISA Kit
products short name :
brain natriuretic peptide, BNP
products name syn :
Human brain natriuretic peptide; BNP ELISA Kit; brain natriuretic peptide; BNP
other names :
brain natriuretic peptide; Brain natriuretic peptide; brain natriuretic peptide; B-type natriuretic peptide
other gene names :
nppb; nppb; bnp; bnp
uniprot entry name :
ANFB_TAKRU
specificity :
This assay has high sensitivity and excellent specificity for detection of Human BNP. No significant cross-reactivity or interference between Human BNP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, urine, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 93.75 pg/ml-6000 pg/ml. Sensitivity: 23.43 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BNP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BNP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001027860.1
ncbi gb acc num :
NM_001032688.1
ncbi mol weight :
14,746 Da
uniprot summary :
Function: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Has a cGMP-stimulating activity
size4 :
10x96-Strip-Wells