ELISA/assay

Human apolipoprotein E, Apo-E ELISA Kit

MBS704520

96-Strip-Wells

630 USD

ELISA Kit

Human apolipoprotein E, Apo-E ELISA Kit

apolipoprotein E

Human apolipoprotein E (Apo-E) ELISA Kit; AD2; LDLCQ5; LPG; MGC1571; apolipoprotein E3; Human apolipoprotein E (Apo-E)

apolipoprotein E; Apolipoprotein E; apolipoprotein E; apo-E; apolipoprotein E3; apolipoprotein E

APOE

APOE; APOE; AD2; LPG; LDLCQ5; Apo-E

APOE_HUMAN

Human

317

This assay has high sensitivity and excellent specificity for detection of Human APOE. No significant cross-reactivity or interference between Human APOE and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Competitive. Detection Range: 15.62 ng/ml-1000 ng/ml. Sensitivity: 3.9ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Apolipoprotein

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apo-E. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Apo-E. The competitive inhibition reaction is launched between with pre-coated Apo-E and Apo-E in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Apo-E in the samples. The color development is stopped and the intensity of the color is measured.

4557325

NP_000032.1

NM_000041.2

P02649

36,154 Da

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156)

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jul 2008]

Function: Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.

96-Strip-Wells

630 USD

5x96-Strip-Wells

1955

10x96-Strip-Wells

3635