catalog number :
MBS704511
products type :
ELISA Kit
products full name :
Rat Gastrin ELISA Kit
products short name :
gastrin
products name syn :
Rat Gastrin ELISA Kit; GAS; ; gastrin
other names :
gastrin; Gastrin; gastrin; gastrin; Gastrin-34; G34
products gene name :
GAST
other gene names :
Gast; Gast; Gas; PPG34; Gas; G34
uniprot entry name :
GAST_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat gastrin. No significant cross-reactivity or interference between rat gastrin and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 pg/ml -20 pg/ml. Sensitivity: 0.078 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for gastrin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any gastrin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for gastrin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of gastrin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_036981.1
ncbi gb acc num :
NM_012849.1
ncbi mol weight :
11,832 Da
ncbi pathways :
G Alpha (q) Signalling Events Pathway (1010197); GPCR Downstream Signaling Pathway (1010193); Gastric Acid Secretion Pathway (154405); Gastric Acid Secretion Pathway (154383); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1010213); Signal Transduction Pathway (1010015); Signaling By GPCR Pathway (1010162)
ncbi summary :
increases gastric mucosal integrity through induction of COX-2 expression [RGD, Feb 2006]
uniprot summary :
Function: Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
size4 :
10x96-Strip-Wells