catalog number :
MBS704510
products type :
ELISA Kit
products full name :
Rat Tissue factor, TF ELISA Kit
products short name :
[coagulation factor III (thromboplastin, tissue factor)]
products name syn :
[Rat Tissue factor; TF ELISA Kit; CD142; TF; TFA; coagulation factor III; tissue factor; coagulation factor III (thromboplastin; tissue factor)]
other names :
[tissue factor; Tissue factor; tissue factor; TF; brain tissue factor; Coagulation factor III; CD_antigen: CD142]
products gene name :
[F3]
other gene names :
[F3; F3; TF]
uniprot entry name :
TF_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat TF. No significant cross-reactivity or interference between rat TF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, urine, cell culture supernates, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 31.2 pg/ml-2000 pg/ml. Sensitivity: The minimum detectable dose of rat TF is typically less than 7.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075760.1
ncbi gb acc num :
NM_001082291.1
ncbi mol weight :
32,738 Da
ncbi pathways :
Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Extrinsic Pathway (106058); Formation Of Fibrin Clot (Clotting Cascade) Pathway (106057); Hemostasis Pathway (106028)
uniprot summary :
Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells