ELISA/assay

Rat B-cell CLL/lymphoma 2, BCL2 ELISA Kit

MBS704498

48-Strip-Wells

605 USD

ELISA Kit

Rat B-cell CLL/lymphoma 2, BCL2 ELISA Kit

B-cell CLL/lymphoma 2

Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit; Bcl-2; B-cell lymphoma protein 2; OTTHUMP00000163680; B-cell CLL/lymphoma 2

apoptosis regulator Bcl-2; Apoptosis regulator Bcl-2; apoptosis regulator Bcl-2; Bcl2-like protein; B-cell leukemia/lymphoma 2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2

BCL2

Bcl2; Bcl2; Bcl-2; Bcl-2

BCL2_RAT

Rat

236

This assay has high sensitivity and excellent specificity for detection of rat BCL2. No significant cross-reactivity or interference between rat BCL2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of rat BCL2 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BCL2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

8392974

NP_058689.1

NM_016993.1

P49950

26,622 Da

ATM Pathway (198524); Adrenergic Signaling In Cardiomyocytes Pathway (908278); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (219785); Apoptosis Pathway (83452); Apoptosis Pathway (470); B Cell Receptor Signaling Pathway (198486); Cholinergic Synapse Pathway (217713)

an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD, Feb 2006]

Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

48-Strip-Wells

605 USD

96-Strip-Wells

870

5x96-Strip-Wells

2950

10x96-Strip-Wells

5535